This study examines using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction approach coupled with either traditional dispersive-Solid Phase Extraction (dSPE) clean up versus UCT’s dSPE clean up in 96-well plate format using Hamilton’s [MPE]2 — automated positive pressure extraction and evaporation module — for the analysis of 47 pesticides in marijuana. This analysis demonstrates that for most compounds investigated, the high-throughput cleanup method exhibits comparable results to traditional dSPE cleanup.