Different Immobilized Method of CRL to Influence on Chiral Resolution of Racemic Paroxol
Different immobilized method of CRL to influence on chiral resolution of racemic paroxol was explored. First of all, physical adsorption, covalent attachment and enzyme-aggregate coating methods were performed to prepare immobilized enzyme. The immobilization efficiencies of immobilized BCLs by physical adsorption, covalent attachment (CA-BCL-NH2-IL-SBA) and enzyme-aggregate coating methods (EAC-BCL-NH2-IL-SBA) were 69%, 67% and 85%, respectively. CA-BCL-NH2-IL-SBA and EAC-BCL-NH2-IL-SBA possessed higher thermal stability, reusability, storage stability than BCL-NH2-IL-SBA. Lastly, three kinds of different immobilized method to influence on chiral resolution of racemic paroxol were anlysised. The enzyme immobilized by enzyme-aggregate coating showed high ee (95%) and E(78). The physical adsorption showed the high conversion(42%).