The Nucleotide Sequence Diversity Analysis of the Pig MyoG Gene
The MyoG gene was sequenced in 63 individuals from 10 breeds (eight Chinese domestic breeds, one recently derived breed and one introduced breed) of pigs in this experiment to analyze the nucleotide sequence diversity of the MyoG gene. The following conclusions can be drawn from the experiment. In the complete sequence of the pig MyoG gene, the content of C+G base is significantly higher than that of A+T. And the high content of G and C base in the coding region caused that bias. In the coding region of the MyoG gene, the G and C base are abundant at the 1st and the 3rd site of the codon, especially at the 3rd site. The high content of G and C base is beneficial for the stability of the MyoG gene. Generally speaking, the MyoG gene is very conservative, the composition and construction of the coding region are very stability, but mutations can be found in noncoding region. Transversion happens with higher frequency than that of transition, and transition happens mainly between T and C base, transversions mainly between G and C base. In the flanking region and intronic region, the MyoG gene has relatively high nucleotide diversity. These mutations exist in noncoding region caused the nucleotide sequence diversity of the MyoG gene.