Notice of RetractionThe Optimization of the Bio-Panning Process of the Affinity Ligand of Tyrosinase by Phage Display Technology
The aim of this study was to search an optimal bio-panning process of phage display. In this study, the target protein is tyrosinase. Through changing the screening conditions, we found a successful method to obtain a high recovery ratio when the specificities of the sequences were not affected. Ultimately, the optimum bio-panning conditions are as follows: the incubate time is 40min, the wash time is 6 times and the wash time is 35min. The verifying experiment showed that this bio-panning process can obtain a high recovery ratio of phages, and the result of the ELISA showed that the specificities of the sequences were not affected.