Atomic force microscope observation on CHO cell membrane after Electromagnetic pulses irradiation
Atomic force microscope (AFM) has been used to visualize the morphological change on the surface of CHO cell membrane before and after Electromagnetic pulses (EMP)irradiation. The results showed that there were different sizes and shapes of membrane perforate (the width is 0.66-0.39 um, the depth is 392.95 nm) after 400 kV/m 200 pulses EMP irradiation. The numbers of cell membrane perforates were decreased with EMP field intensity and the pulses numbers decreasing. It is suggested that EMP irradiation can cause the membrane perforate of CHO cell directly. The numbers of cell membrane perforates were increased with EMP field intensity and the pulses numbers increasing. EMP induced membrane perforate may play a very important role in EMP bio-effects.