Biophysical measurements in 2D and 3D live cell imaging using fluid dynamics and optical flow
Measuring the biophysical properties of living cells is essential to understand their underlying cytoskeletal machinery. Here we describe a robust extension of our previous algorithm for the intra-cellular measurement of pressure, velocity and forces inside living cells. We first consider a compressible fluid model to cope with out-of-plane intracellular flow in the 2D case, and also propose a 3D version of the algorithm for multi-plane imaging. Results demonstrate the high potential of the approach, giving access to biophysical measures that cannot be achieved using other experimental techniques.