Notice of RetractionDetermination of Thiourea by High Performance Liquid Chromatography with Different Solvents
Thiourea is a toxicant which will brings some adverse affects to the public health, even in some cases is fatal. The detection of thiourea by High Performance Liquid Chromatography (HPLC) on different solvents, as methanol, ethanol and ultrapure water were studied respectively. The results showed that the concentrations between 0.5 μ g/mL and 5.0 μ g/mL, the peak area of thiourea in three different solvents had a good relationship with its solution concentrations, the correlation factor were all above 0.98, under the same chromatogram condition as 40% methanol and 60%ultrapure water as the mobile phase, flow rate of the mobile phase was 0.6mL/min, UV-VIS detector at 242nm, column oven was at 30°C, sample size was in 10μL, When used the ultrapure water as the solvent, the relative standard deviation (RSD) ranged from 1.19% to 2.21%, the recovery rate was 88.9%, which demonstrated the method was correct and reliable, and accuracy rating was high.