Novel Affinity Membrane Chromatography Technology: Purification of Pepsin Using Immobilized Reactive Dye Ligands
Novel membranes were prepared through covalently coupling of chitosan (CS) to activate nylon membrane after the reaction of the microporous nylon membrane with formaldehyde. Non-specific adsorption on the CS-coated nylon membrane decreased greatly. Yellow 3G-P/Reactive Red 120 were then covalently immobilized onto composite membrane as dye ligands. These Red 120/Yellow 3G-P-CS-membranes were used in the Pepsin adsorption studies. According to the results, it appears that Yellow 3G-P-CS-nylon membranes could be applied for separation and purification of Pepsin without causing any denaturation, much better than Red 120-CS-nylon membrane. Keywords-affinity chromatography; pepsin; adsorption;