The Bovine Serum Albumin role's in analyzing the its of soil fungus
In order to analyze the Bovine Serum Albumin role's in ITS-PCR technique. We observed the improvement of ITS-PCR application through added BSA in PCR systems. The result shown: In fungi ITS-PCR analysis process, adding BSA should improve the ITS-PCR amplification effect notably and reduce the amount of Taq enzymes, the best use concentration of BSA of 2 μ g/μ l. This suggested: the BSA adding to improve soil fungus ITS-PCR analysis method was feasible; The study provided the basis for applying BSA in soil fungus ITS-PCR analysis technology.